Journal: Journal of Neuroinflammation
Article Title: Metabolic reprogramming and lipid droplets are involved in Zika virus replication in neural cells
doi: 10.1186/s12974-023-02736-7
Figure Lengend Snippet: Inhibition of lipid droplet accumulation induced by ZIKV decreases viral replication in neuroblastoma cells. A Representative images of SH-SY5Y cells 48 h after ZIKV infection treated with 50 µM DGAT-1 inhibitor (A922500) and stained with Oil Red O (red) and DAPI (blue) for nuclei. The scale bar represents 10 µm in range. B Quantification of the fluorescent area per cell in each group. C Real-time PCR for PLIN-2 after 24 h of ZIKV infection and treatment with a DGAT-1 inhibitor. D Effect of the DGAT-1 inhibitor on ZIKV replication at 48 hpi. E Images of SH-SY5Y cells after 48 h of ZIKV infection and the ds-RNA stained with J2 antibody (green), the lipid droplets were stained with LipidTox (Red) and nuclei with DAPI (blue). The scale bar represents 10 µm in range. F Quantification of LD pixels per cell. G Quantification of ds-RNA pixels per cell. Data information: In B – D , F , G ), data are presented as the means ± SEMs of three independent experiments. * P < 0.05 mock- versus ZIKV-infected cells. # P < 0.05 ZIKV-infected cells versus A922500 treatments
Article Snippet: The cDNA was amplified in 10 μl of 1 × TaqMan universal PCR master mix with Predeveloped TaqMan assay primers and probes Perilipin-2 (PLIN-2), Hs00605340_m1; diacylglycerol O-acyltransferase 1 (DGAT-1), Hs01020362_g1; fatty acid synthase (FASN) Hs01005622_m1 and beta-actin (ACTB) Hs99999903_m1 as endogenous controls (Thermo Fisher Scientific) according to the manufacturer's instructions.
Techniques: Inhibition, Infection, Staining, Real-time Polymerase Chain Reaction
Proteintech is a verified supplier 
